Strong Immune Responses Induced by Direct Local Injections of Modified mRNA-Lipid Nanocomplexes.

ABSTRACT: In vitro transcribed mRNAs hold the promises of many medical applications in disease prevention and treatment, such as replacement or supplement of missing or inadequately expressed endogenous proteins and as preventive vaccines against infectious diseases, therapeutic vaccines, or other protein-based biopharmaceutics for cancer therapy. A safe and efficient delivery system for mRNA is crucial to the success of mRNA therapeutic applications. In this study, we report that InstantFECT, a liposome-based transfection reagent, can pack pseudouridine-incorporated mRNA into nanocomplexes that are highly efficient in mediating in vivo transfection in multiple organs after local delivery. High levels of expression of EGFP and luciferase reporters after intratumoral and intramuscular injections were

SUBMITTER: Arya S 

PROVIDER: S-EPMC7016160 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature