Nanoscale Remodeling of Functional Synaptic Vesicle Pools in Hebbian Plasticity.

ABSTRACT: Vesicle pool properties are known determinants of synaptic efficacy, but their potential role as modifiable substrates in forms of Hebbian plasticity is still unclear. Here, we investigate this using a nanoscale readout of functionally recycled vesicles in natively wired hippocampal CA3→CA1 circuits undergoing long-term potentiation (LTP). We show that the total recycled vesicle pool is larger after plasticity induction, with the smallest terminals exhibiting the greatest relative expansion. Changes in the spatial organization of vesicles accompany potentiation including a specific increase in the number of recycled vesicles at the active zone, consistent with an ultrastructural remodeling component of synaptic strengthening. The cAMP-PKA pathway activator, forskolin, selectively mimics so

SUBMITTER: Rey S 

PROVIDER: S-EPMC7016504 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature