In-Depth Mapping of the Urinary N-Glycoproteome: Distinct Signatures of ccRCC-related Progression.

ABSTRACT: Protein N-glycosylation is one of the most important post-translational modifications and is involved in many biological processes, with aberrant changes in protein N-glycosylation patterns being closely associated with several diseases, including the progression and spreading of tumours. In light of this, identifying these aberrant protein glycoforms in tumours could be useful for understanding the molecular mechanism of this multifactorial disease, developing specific biomarkers and finding novel therapeutic targets. We investigated the urinary N-glycoproteome of clear cell renal cell carcinoma (ccRCC) patients at different stages (n = 15 at pT1 and n = 15 at pT3), and of non-ccRCC subjects (n = 15), using an N-glyco-FASP-based method. Using label-free nLC-ESI MS/MS, we identified and qu

SUBMITTER: Santorelli L 

PROVIDER: S-EPMC7016614 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature