Off Earth Identification of Bacterial Populations Using 16S rDNA Nanopore Sequencing.

ABSTRACT: The MinION sequencer has made in situ sequencing feasible in remote locations. Following our initial demonstration of its high performance off planet with Earth-prepared samples, we developed and tested an end-to-end, sample-to-sequencer process that could be conducted entirely aboard the International Space Station (ISS). Initial experiments demonstrated the process with a microbial mock community standard. The DNA was successfully amplified, primers were degraded, and libraries prepared and sequenced. The median percent identities for both datasets were 84%, as assessed from alignment of the mock community. The ability to correctly identify the organisms in the mock community standard was comparable for the sequencing data obtained in flight and on the ground. To validate the process on

SUBMITTER: Burton AS 

PROVIDER: S-EPMC7016637 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature