A yeast display immunoprecipitation screen for targeted discovery of antibodies against membrane protein complexes.

ABSTRACT: Yeast display immunoprecipitation is a combinatorial library screening platform for the discovery and engineering of antibodies against membrane proteins using detergent-solubilized membrane fractions or cell lysates as antigen sources. Here, we present the extension of this method for the screening of antibodies that bind to membrane protein complexes, enabling discovery of antibodies that target antigens involved in a functional protein-protein interaction of interest. For this proof-of-concept study, we focused on the receptor-mediated endocytosis machinery at the blood-brain barrier, and adaptin 2 (AP-2) was chosen as the functional interaction hub. The goal of this study was to identify antibodies that bound to blood-brain barrier (BBB) membrane protein complexes containing AP-2. Scre

SUBMITTER: Lajoie JM 

PROVIDER: S-EPMC7017056 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature