Can-Seq: a PCR and DNA sequencing strategy for identifying new alleles of known and candidate genes.

ABSTRACT:

Background

Forward genetic screens are a powerful approach for identifying the genes contributing to a trait of interest. However, mutants arising in genes already known can obscure the identification of new genes contributing to the trait. Here, we describe a strategy called Candidate gene-Sequencing (Can-Seq) for rapidly identifying and filtering out mutants carrying new alleles of known and candidate genes.

Results

We carried out a forward genetic screen and identified 40 independent Arabidopsis mutants with defects in systemic spreading of RNA interference (RNAi), or more specifically in root-to-shoot transmission of post-transcriptional gene silencing (rtp). To classify the mutants as either representing a new allele of a known or candidate g

SUBMITTER: Cao J 

PROVIDER: S-EPMC7017465 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature