CtpB is a plasma membrane copper (I) transporting P-type ATPase of Mycobacterium tuberculosis.

ABSTRACT:

Background

The intracellular concentration of heavy-metal cations, such as copper, nickel, and zinc is pivotal for the mycobacterial response to the hostile environment inside macrophages. To date, copper transport mediated by P-type ATPases across the mycobacterial plasma membrane has not been sufficiently explored.

Results

In this work, the ATPase activity of the putative Mycobacterium tuberculosis P_1B-type ATPase CtpB was associated with copper (I) transport from mycobacterial cells. Although CtpB heterologously expressed in M. smegmatis induced tolerance to toxic concentrations of Cu²⁺ and a metal preference for Cu⁺, the disruption of ctpB in M. tuberculosis cells did not promote impaired cell growth or heavy-metal accumulation in whole m

SUBMITTER: Leon-Torres A 

PROVIDER: S-EPMC7017476 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature