Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression.

ABSTRACT:

Background

Nuclear translocation of several oncogenic proteins have previously been reported, but neither the translocation of doublecortin (DCX) nor the mechanism involved has been studied. DCX is a neuronal microtubule-associated protein (MAP) that is crucial for adult neurogenesis and neuronal migration and has been associated with poor prognosis in gliomas.

Methods

We probed DCX expression in different grades of glioma tissues and conventional cells via western blotting. Then we analyzed the expression pattern in the Oncomine cancer profiling database. Confocal Immunofluorescence was used to detect DCX expression in the cellular compartments, while subcellular fractionation was probed via western blotting. Pulse shape height analysis was utilized to verify DCX localizati

SUBMITTER: Ayanlaja AA 

PROVIDER: S-EPMC7017634 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature