Proteome Dynamics from Heavy Water Metabolic Labeling and Peptide Tandem Mass Spectrometry.

ABSTRACT: Protein homeostasis (proteostasis) is a result of a dynamic equilibrium between protein synthesis and degradation. It is important for healthy cell/organ functioning and is often associated with diseases such as neurodegenerative diseases and non-Alcoholic Fatty Liver disease. Heavy water metabolic labeling, combined with liquid-chromatography and mass spectrometry (LC-MS), is a powerful approach to study proteostasis in vivo in high throughput. Traditionally, intact peptide signals are used to estimate stable isotope incorporation in time-course experiments. The time-course of label incorporation is used to extract protein decay rate constant (DRC). Intact peptide signals, computed from integration in chromatographic time and mass-to-charge ratio (m/z) domains, usually, provide an accurat

SUBMITTER: Borzou A 

PROVIDER: S-EPMC7017751 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature