Novel Engraftment and T Cell Differentiation of Human Hematopoietic Cells in ART^-/-IL2RG^-/Y SCID Pigs.

ABSTRACT: Pigs with severe combined immunodeficiency (SCID) are an emerging biomedical animal model. Swine are anatomically and physiologically more similar to humans than mice, making them an invaluable tool for preclinical regenerative medicine and cancer research. One essential step in further developing this model is the immunological humanization of SCID pigs. In this work we have generated T^- B^- NK^- SCID pigs through site directed CRISPR/Cas9 mutagenesis of IL2RG within a naturally occurring DCLRE1C (ARTEMIS)^-/- genetic background. We confirmed ART^-/-IL2RG^-/Y pigs lacked T, B, and NK cells in both peripheral blood and lymphoid tissues. Additionally, we successfully performed a bone marrow trans

SUBMITTER: Boettcher AN 

PROVIDER: S-EPMC7017803 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature