Raman-deuterium isotope probing to study metabolic activities of single bacterial cells in human intestinal microbiota.

ABSTRACT: Human intestinal microbiota is important to host health and is associated with various diseases. It is a challenge to identify the functions and metabolic activity of microorganisms at the single-cell level in gut microbial community. In this study, we applied Raman microspectroscopy and deuterium isotope probing (Raman-DIP) to quantitatively measure the metabolic activities of intestinal bacteria from two individuals and analysed lipids and phenylalanine metabolic pathways of functional microorganisms in situ. After anaerobically incubating the human faeces with heavy water (D₂ O), D₂ O with specific substrates (glucose, tyrosine, tryptophan and oleic acid) and deuterated glucose, the C-D band in single-cell Raman spectra appeared in some bacteria in faeces, due to t

SUBMITTER: Wang Y 

PROVIDER: S-EPMC7017835 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature