Microbial resolution of whole genome shotgun and 16S amplicon metagenomic sequencing using publicly available NEON data.

ABSTRACT: Microorganisms are ubiquitous in the biosphere, playing a crucial role in both biogeochemistry of the planet and human health. However, identifying these microorganisms and defining their function are challenging. Widely used approaches in comparative metagenomics, 16S amplicon sequencing and whole genome shotgun sequencing (WGS), have provided access to DNA sequencing analysis to identify microorganisms and evaluate diversity and abundance in various environments. However, advances in parallel high-throughput DNA sequencing in the past decade have introduced major hurdles, namely standardization of methods, data storage, reproducible interoperability of results, and data sharing. The National Ecological Observatory Network (NEON), established by the National Science Foundation, enables al

SUBMITTER: Brumfield KD 

PROVIDER: S-EPMC7018008 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature