Differential processing of high-molecular-weight kininogen during normal pregnancy.

ABSTRACT:

Rationale

Studies identified kininogen as a potential biomarker of preeclampsia, a major cause of adverse maternal outcomes. High-molecular-weight kininogen (HK) and its activated form participate in numerous pathways associated with establishing and maintaining pregnancy. However, dynamic changes in HK and naturally occurring HK-derived peptides during the natural course of pregnancy are largely unknown.

Methods

Longitudinal serum samples during the course of normal pregnancy (trimesters T1, T2, T3) from 60 pregnant women were analyzed by western blot with an anti-HK antibody. Circulating peptides in longitudinal serum specimens derived from 50 participants were enriched using nanoporous silica thin films. Peptides were identified by liquid chromatography/tandem mass spectr

SUBMITTER: Droll SH 

PROVIDER: S-EPMC7018535 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature