Characterization of TP53 mutations in Pap test DNA of women with and without serous ovarian carcinoma.

ABSTRACT:

Objective

Pap tests hold promise as a molecular diagnostic for serous ovarian cancer, but previous studies reported limited sensitivity. Furthermore, the presence of somatic mutations in normal tissue is increasingly recognized as a challenge to the specificity of mutation-based cancer diagnostics. We applied an ultra-deep sequencing method with the goal of improving sensitivity and characterizing the landscape of low-frequency somatic TP53 mutations in Pap tests.

Methods

We used CRISPR-DS to deeply sequence (mean Duplex depth ~3000×) the TP53 gene in 30 Pap tests from 21 women without cancer and 9 women with serous ovarian carcinoma with known TP53 driver mutations. Mutations were annotated and compared to those in the TP53 cancer database.

Results

The tumor-derived

SUBMITTER: Krimmel-Morrison JD 

PROVIDER: S-EPMC7018621 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature