Characterisation of the developing heart in a pressure overloaded model utilising RNA sequencing to direct functional analysis.

ABSTRACT: Cardiogenesis is influenced by both environmental and genetic factors, with blood flow playing a critical role in cardiac remodelling. Perturbation of any of these factors could lead to abnormal heart development and hence the formation of congenital heart defects. Although abnormal blood flow has been associated with a number of heart defects, the effects of abnormal pressure load on the developing heart gene expression profile have to date not clearly been defined. To determine the heart transcriptional response to haemodynamic alteration during development, outflow tract (OFT) banding was employed in the chick embryo at Hamburger and Hamilton stage (HH) 21. Stereological and expression studies, including the use of global expression analysis by RNA sequencing with an optimised procedure

SUBMITTER: Parnall M 

PROVIDER: S-EPMC7018637 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature