Plasmids Shaped the Recent Emergence of the Major Nosocomial Pathogen Enterococcus faecium.

ABSTRACT: Enterococcus faecium is a gut commensal of humans and animals but is also listed on the WHO global priority list of multidrug-resistant pathogens. Many of its antibiotic resistance traits reside on plasmids and have the potential to be disseminated by horizontal gene transfer. Here, we present the first comprehensive population-wide analysis of the pan-plasmidome of a clinically important bacterium, by whole-genome sequence analysis of 1,644 isolates from hospital, commensal, and animal sources of E. faecium Long-read sequencing on a selection of isolates resulted in the completion of 305 plasmids that exhibited high levels of sequence modularity. We further investigated the entirety of all plasmids of each isolate (plasmidome) using a combination of short-read sequencing and

SUBMITTER: Arredondo-Alonso S 

PROVIDER: S-EPMC7018651 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature