Otoferlin C2F Domain-Induced Changes in Membrane Structure Observed by Sum Frequency Generation.

ABSTRACT: Proteins that contain C2 domains are involved in a variety of biological processes, including encoding of sound, cell signaling, and cell membrane repair. Of particular importance is the interface activity of the C-terminal C2F domain of otoferlin due to the pathological mutations known to significantly disrupt the protein's lipid membrane interface binding activity, resulting in hearing loss. Therefore, there is a critical need to define the geometry and positions of functionally important sites and structures at the otoferlin-lipid membrane interface. Here, we describe the first in situ probe of the protein orientation of otoferlin's C2F domain interacting with a cell membrane surface. To identify this protein's orientation at the lipid interface, we applied sum frequency generation (SFG

SUBMITTER: Golbek TW 

PROVIDER: S-EPMC7019022 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature