Megakaryocytes promote bone formation through coupling osteogenesis with angiogenesis by secreting TGF-β1.

ABSTRACT: Rationale: The hematopoietic system and skeletal system have a close relationship, and megakaryocytes (MKs) may be involved in maintaining bone homeostasis. However, the exact role and underlying mechanism of MKs in bone formation during steady-state and stress conditions are still unclear. Methods: We first evaluated the bone phenotype with MKs deficiency in bone marrow by using c-Mpl-deficient mice and MKs-conditionally deleted mice. Then, osteoblasts (OBs) proliferation and differentiation and CD31^hiEmcn^hi tube formation were assessed. The expression of growth factors related to bone formation in MKs was detected by RNA-sequencing and enzyme-linked immunosorbent assays (ELISAs). Mice with specific depletion of TGF-β1 in MKs were used to further verify

SUBMITTER: Tang Y 

PROVIDER: S-EPMC7019172 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature