Tracing Baculovirus AcMNPV Infection Using a Real-Time Method Based on ANCHOR^TM DNA Labeling Technology.

ABSTRACT: Many steps in the baculovirus life cycle, from initial ingestion to the subsequent infection of all larval cells, remain largely unknown; primarily because it has hitherto not been possible to follow individual genomes and their lineages. Use of ANCHOR^TM technology allows a high intensity fluorescent labelling of DNA. When applied to a virus genome, it is possible to follow individual particles, and the overall course of infection. This technology has been adapted to enable labelling of the baculovirus Autographa californica Multiple NucleoPolyhedroVirus genome, as a first step to its application to other baculoviruses. AcMNPV was modified by inserting the two components of ANCHOR^TM: a specific DNA-binding protein fused to a fluorescent reporter, and the corresponding

SUBMITTER: Hinsberger A 

PROVIDER: S-EPMC7019957 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature