DENV2 Pseudoviral Particles with Unprocessed Capsid Protein Are Assembled and Infectious.

ABSTRACT: Proteolytic processing of flavivirus polyprotein is a uniquely controlled process. To date, the sequential cleavage of the capsid anchor sequence at the junction of C-PrM has been considered essential for the production of flaviviruses. In this study, we used two experimental approaches to show the effect of unprocessed capsid on the production and infectivity of dengue virus 2 (DENV2) pseudoviral particles. The results showed that (1) both mature and unprocessed capsids of DENV2 were equally efficient in the viral RNA packaging and also in the assembly of infective particles; (2) DENV2 variants, in which the viral and host mediated cleavage of Ca peptide were independent, produced significantly higher levels of infective particles. Overall, this study demonstrated that unlike other flaviv

SUBMITTER: Rana J 

PROVIDER: S-EPMC7019998 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature