Inflammasome Activation Induced by a Snake Venom Lys49-Phospholipase A₂ Homologue.

ABSTRACT:

Background

Snake venom phospholipases A₂ (PLA₂s) have hemolytic, anticoagulant, myotoxic, oedematogenic, bactericidal, and inflammatory actions. BthTX-I, a Lys49-PLA₂ isolated from Bothrops jararacussu venom, is an example of Lys49-PLA₂ that presents such actions. NLRP3 is a cytosolic receptor from the NLR family responsible for inflammasome activation via caspase-1 activation and IL-1β liberation. The study of NLRs that recognize tissue damage and activate the inflammasome is relevant in envenomation.

Methods

Male mice (18-20 g) received an intramuscular injection of BthTX-I or sterile saline. The serum was collected for creatine-kinase (CK), lactate dehydrogenase (LDH), and interleukin-1β (IL-1β) assays, and muscle was rem

SUBMITTER: Boeno CN 

PROVIDER: S-EPMC7020408 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature