Calcium-Induced Activity and Folding of a Repeat in Toxin Lipase from Antarctic Pseudomonas fluorescens Strain AMS8.

ABSTRACT: It is hypothesized that the Ca²⁺ ions were involved in the activity, folding and stabilization of many protein structures. Many of these proteins contain repeat in toxin (RTX) motifs. AMS8 lipase from Antarctic Pseudomonas fluorescens strain AMS8 was found to have three RTX motifs. So, this research aimed to examine the influence of Ca²⁺ ion towards the activity and folding of AMS8 lipase through various biophysical characterizations. The results showed that CaCl₂ increased lipase activity. The far-UV circular dichroism (CD) and Fourier-transform infrared (FTIR) analysis suggested that the secondary structure content was improved with the addition of CaCl₂. Fluorescence spectroscopy analysis showed that the presence of CaCl₂ inc

SUBMITTER: Ali NSM 

PROVIDER: S-EPMC7020413 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature