Influence of inflammatory conditions provided by macrophages on osteogenic ability of mesenchymal stem cells.

ABSTRACT:

Background

The mechanisms by which macrophage phenotype contributes to mesenchymal stem cells (MSC)-mediated bone repair remain unclear. In this work, we investigated the influence of factors released by human macrophages polarized to a pro-inflammatory or an anti-inflammatory phenotype on the ability of human MSC to attach, migrate, and differentiate toward the osteoblastic lineage. We focused on the role of TNF-α and IL-10, key pro-inflammatory and anti-inflammatory cytokines, respectively, in regulating MSC functions.

Methods

MSC were treated with media conditioned by pro-inflammatory or anti-inflammatory macrophages to study their influence in cell attachment, migration, and osteogenic differentiation. The involvement of TNF-α and IL-10 in the regulation of MSC functions

SUBMITTER: Valles G 

PROVIDER: S-EPMC7020593 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature