The nociceptive innervation of the normal and osteoarthritic mouse knee.

ABSTRACT:

Objectives

To document the nociceptive innervation of the normal and osteoarthritic murine knee.

Methods

Knees were collected from naïve male C57BL/6 Na_V1.8-tdTomato reporter mice aged 10, 26, and 52 weeks (n = 5/group). Destabilization of the medial meniscus (DMM) or sham surgeries (n = 5/group) were performed in the right knee of 10-week old male Na_V1.8-tdTomato mice, and knees were harvested 16 weeks later. Twenty 20-μm frozen sections from a 400-μm mid-joint region were collected for confocal microscopy. Integrated density of the tdTomato signal was calculated using Image J by two independent observers blinded to the groups. Consecutive sections were stained with hematoxylin & eosin. C57BL/6-Pirt-GCaMP3 mice (n = 5/group) and protein gene product 9.

SUBMITTER: Obeidat AM 

PROVIDER: S-EPMC7020657 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature