Comprehensive transcriptome analysis of reference genes for fruit development of Euscaphis konishii.

ABSTRACT:

Background

Quantitativereal-time reverse transcriptase polymerase chain reaction is the common method to quantify relative gene expression. Normalizating using reliable genes is critical in correctly interpreting expression data from qRT-PCR. Euscaphis konishii is a medicinal plant with a long history in China, which has various chemical compounds in fruit. However, there is no report describing the selection of reference genes in fruit development of Euscaphis konishii.

Methods

We selected eight candidate reference genes based on RNA-seq database analysis, and ranked expression stability using statistical algorithms GeNorm, NormFinder, BestKeeper and ReFinder. Finally, The nine genes related to the anthocyanin synthesis pathway of Euscaphis konishii were used

SUBMITTER: Yang CL 

PROVIDER: S-EPMC7020815 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature