Structure of galactarate dehydratase, a new fold in an enolase involved in bacterial fitness after antibiotic treatment.

ABSTRACT: Galactarate dehydratase (GarD) is the first enzyme in the galactarate/glucarate pathway and catalyzes the dehydration of galactarate to 3-keto-5-dehydroxygalactarate. This protein is known to increase colonization fitness of intestinal pathogens in antibiotic-treated mice and to promote bacterial survival during stress. The galactarate/glucarate pathway is widespread in bacteria, but not in humans, and thus could be a target to develop new inhibitors for use in combination therapy to combat antibiotic resistance. The structure of almost all the enzymes of the galactarate/glucarate pathway were solved previously, except for GarD, for which only the structure of the N-terminal domain was determined previously. Herein, we report the first crystal structure of full-length GarD solved using a s

SUBMITTER: Rosas-Lemus M 

PROVIDER: S-EPMC7021002 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature