A Universal, Genomewide GuideFinder for CRISPR/Cas9 Targeting in Microbial Genomes.

ABSTRACT: The CRISPR/Cas system has significant potential to facilitate gene editing in a variety of bacterial species. CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) represent modifications of the CRISPR/Cas9 system utilizing a catalytically inactive Cas9 protein for transcription repression and activation, respectively. While CRISPRi and CRISPRa have tremendous potential to systematically investigate gene function in bacteria, few programs are specifically tailored to identify guides in draft bacterial genomes genomewide. Furthermore, few programs offer open-source code with flexible design parameters for bacterial targeting. To address these limitations, we created GuideFinder, a customizable, user-friendly program that can design guides for any annotated bacterial genome. GuideFin

SUBMITTER: Spoto M 

PROVIDER: S-EPMC7021469 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature