Tobacco smoking and somatic mutations in human bronchial epithelium.

ABSTRACT: Tobacco smoking causes lung cancer^1-3, a process that is driven by more than 60 carcinogens in cigarette smoke that directly damage and mutate DNA^4,5. The profound effects of tobacco on the genome of lung cancer cells are well-documented^6-10, but equivalent data for normal bronchial cells are lacking. Here we sequenced whole genomes of 632 colonies derived from single bronchial epithelial cells across 16 subjects. Tobacco smoking was the major influence on mutational burden, typically adding from 1,000 to 10,000 mutations per cell; massively increasing the variance both within and between subjects; and generating several distinct mutational signatures of substitutions and of insertions and deletions. A population of cells in individuals with a history of sm

SUBMITTER: Yoshida K 

PROVIDER: S-EPMC7021511 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature