Live-animal imaging of native haematopoietic stem and progenitor cells.

ABSTRACT: The biology of haematopoietic stem cells (HSCs) has predominantly been studied under transplantation conditions^1,2. It has been particularly challenging to study dynamic HSC behaviour, given that the visualization of HSCs in the native niche in live animals has not, to our knowledge, been achieved. Here we describe a dual genetic strategy in mice that restricts reporter labelling to a subset of the most quiescent long-term HSCs (LT-HSCs) and that is compatible with current intravital imaging approaches in the calvarial bone marrow^3-5. We show that this subset of LT-HSCs resides close to both sinusoidal blood vessels and the endosteal surface. By contrast, multipotent progenitor cells (MPPs) show greater variation in distance from the endosteum and are more likely to b

SUBMITTER: Christodoulou C 

PROVIDER: S-EPMC7021587 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature