CEP44 ensures the formation of bona fide centriole wall, a requirement for the centriole-to-centrosome conversion.

ABSTRACT: Centrosomes are essential organelles with functions in microtubule organization that duplicate once per cell cycle. The first step of centrosome duplication is the daughter centriole formation followed by the pericentriolar material recruitment to this centriole. This maturation step was termed centriole-to-centrosome conversion. It was proposed that CEP295-dependent recruitment of pericentriolar proteins drives centriole conversion. Here we show, based on the analysis of proteins that promote centriole biogenesis, that the developing centriole structure helps drive centriole conversion. Depletion of the luminal centriole protein CEP44 that binds to the A-microtubules and interacts with POC1B affecting centriole structure and centriole conversion, despite CEP295 binding to centrioles. Impa

SUBMITTER: Atorino ES 

PROVIDER: S-EPMC7021698 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature