A Novel in situ Approach to Studying Detrusor Smooth Muscle Cells in Mice.

ABSTRACT: The aim of our study was to develop a novel approach to investigating mouse detrusor smooth muscle cell (SMC) physiological activity, utilizing an acute tissue dissection technique and confocal calcium imaging. The bladder of a sacrificed adult female NMRI mouse was dissected. We used light and transmission electron microscopy to assess morphology of SMCs within the tissue. Calcium imaging in individual SMCs was performed using confocal microscopy during stimulation with increasing concentrations of carbamylcholine (CCh). SMCs were identified according to their morphology and calcium activity. We determined several parameters describing the SMC responses: delays to response, recruitment, relative activity, and contraction of the tissue. CCh stimulation revealed three different SMC phenotyp

SUBMITTER: Serdinsek T 

PROVIDER: S-EPMC7021722 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature