In situ dissection of domain boundaries affect genome topology and gene transcription in Drosophila.

ABSTRACT: Chromosomes are organized into high-frequency chromatin interaction domains called topologically associating domains (TADs), which are separated from each other by domain boundaries. The molecular mechanisms responsible for TAD formation are not yet fully understood. In Drosophila, it has been proposed that transcription is fundamental for TAD organization while the participation of genetic sequences bound by architectural proteins (APs) remains controversial. Here, we investigate the contribution of domain boundaries to TAD organization and the regulation of gene expression at the Notch gene locus in Drosophila. We find that deletion of domain boundaries results in TAD fusion and long-range topological defects that are accompanied by loss of APs and RNA Pol II chromatin binding as well as

SUBMITTER: Arzate-Mejia RG 

PROVIDER: S-EPMC7021724 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature