Structural basis of mammalian high-mannose N-glycan processing by human gut Bacteroides.

ABSTRACT: The human gut microbiota plays a central role not only in regulating the metabolism of nutrients but also promoting immune homeostasis, immune responses and protection against pathogen colonization. The genome of the Gram-negative symbiont Bacteroides thetaiotaomicron, a dominant member of the human intestinal microbiota, encodes polysaccharide utilization loci PULs, the apparatus required to orchestrate the degradation of a specific glycan. EndoBT-3987 is a key endo-β-N-acetylglucosaminidase (ENGase) that initiates the degradation/processing of mammalian high-mannose-type (HM-type) N-glycans in the intestine. Here, we provide structural snapshots of EndoBT-3987, including the unliganded form, the EndoBT-3987-Man₉GlcNAc₂Asn substrate complex, and two EndoBT-3987-Man

SUBMITTER: Trastoy B 

PROVIDER: S-EPMC7021837 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature