Reassessing enzyme kinetics: Considering protease-as-substrate interactions in proteolytic networks.

ABSTRACT: Enzymes are catalysts in biochemical reactions that, by definition, increase rates of reactions without being altered or destroyed. However, when that enzyme is a protease, a subclass of enzymes that hydrolyze other proteins, and that protease is in a multiprotease system, protease-as-substrate dynamics must be included, challenging assumptions of enzyme inertness, shifting kinetic predictions of that system. Protease-on-protease inactivating hydrolysis can alter predicted protease concentrations used to determine pharmaceutical dosing strategies. Cysteine cathepsins are proteases capable of cathepsin cannibalism, where one cathepsin hydrolyzes another with substrate present, and misunderstanding of these dynamics may cause miscalculations of multiple proteases working in one proteolytic n

SUBMITTER: Ferrall-Fairbanks MC 

PROVIDER: S-EPMC7022172 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature