Mapping proteome-wide targets of protein kinases in plant stress responses.

ABSTRACT: Protein kinases are major regulatory components in almost all cellular processes in eukaryotic cells. By adding phosphate groups, protein kinases regulate the activity, localization, protein-protein interactions, and other features of their target proteins. It is known that protein kinases are central components in plant responses to environmental stresses such as drought, high salinity, cold, and pathogen attack. However, only a few targets of these protein kinases have been identified. Moreover, how these protein kinases regulate downstream biological processes and mediate stress responses is still largely unknown. In this study, we introduce a strategy based on isotope-labeled in vitro phosphorylation reactions using in vivo phosphorylated peptides as substrate pools and apply this stra

SUBMITTER: Wang P 

PROVIDER: S-EPMC7022181 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature