Amphiphilic Polypeptides for VEGF siRNA Delivery into Retinal Epithelial Cells.

ABSTRACT: Polyethyleneimine, poly-L-lysine, chitosan and some others cationic polymers have been thoroughly studied as nucleic acid delivery systems in gene therapy. However, the drug release from these systems proceeds at a very low rate due to extremely high binding between a carrier and gene material. To reduce these interactions and to enhance drug release, we developed a set of amphiphilic polypeptides containing positively and negatively charged amino acids as well as a hydrophobic one. The copolymers obtained were characterized by size-exclusion chromatography, static light scattering, HPLC amino acid analysis and 1HNMR spectroscopy. All copolymers formed particles due to a self-assembly in aqueous media. Depending on polypeptide composition, the formation of particles with hydrodynamic diame

SUBMITTER: Osipova O 

PROVIDER: S-EPMC7022581 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature