Silica-Coated Magnetic Iron Oxide Nanoparticles Grafted onto Graphene Oxide for Protein Isolation.

ABSTRACT: In this study, silica-coated magnetic iron oxide nanoparticles (MNPs@SiO₂) were covalently conjugated onto graphene oxide (GO/MNP@SiO₂) for protein isolation. First, MNPs were precisely coated with a silica layer on the surface by using the reverse microemulsion method, followed by incubation with 3-glycidyloxypropyltrimethoxysilane (GPTS) to produce the GPTS-functionalized MNPs@SiO₂ (GPTS-coated MNPs@SiO₂) that display epoxy groups on the surface. The silica shell on the MNPs was optimized at 300 µL of Igepal^®CO-520, 5 mg of MNP, 100 µL of TEOS, 100 µL of NH₄OH and 3% of 3-glycidyloxypropyltrimethoxysilane (GPTS). Simultaneously, polyethyleneimine (PEI) was covalently conjugated to GO to enhance the stability of GO in aqueo

SUBMITTER: Pham XH 

PROVIDER: S-EPMC7022723 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature