In Vitro Autonomous Construction of the Flagellar Axial Structure in Inverted Membrane Vesicles.

ABSTRACT: The bacterial flagellum is a filamentous organelle extending from the cell surface. The axial structure of the flagellum consists of the rod, hook, junction, filament, and cap. The axial structure is formed by axial component proteins exported via a specific protein export apparatus in a well-regulated manner. Although previous studies have revealed the outline of the flagellar construction process, the mechanism of axial structure formation, including axial protein export, is still obscure due to difficulties in direct observation of protein export and assembly in vivo. We recently developed an in vitro flagellar protein transport assay system using inverted membrane vesicles (IMVs) and succeeded in reproducing the early stage of flagellar assembly. However, the late stage of the flagella

SUBMITTER: Terashima H 

PROVIDER: S-EPMC7022808 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature