General and Genomic DNA-Binding Specificity for the <i>Thermus thermophilus</i> HB8 Transcription Factor TTHB023.

ABSTRACT: Transcription factors are proteins that recognize specific DNA sequences and affect local transcriptional processes. They are the primary means by which all organisms control specific gene expression. Understanding which DNA sequences a particular transcription factor recognizes provides important clues into the set of genes that they regulate and, through this, their potential biological functions. Insights may be gained through homology searches and genetic means. However, these approaches can be misleading, especially when comparing distantly related organisms or in cases of complicated transcriptional regulation. In this work, we used a biochemistry-based approach to determine the spectrum of DNA sequences specifically bound by the Thermus thermophilus HB8 TetR-family transcript

SUBMITTER: Shell Cox J 

PROVIDER: S-EPMC7022988 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature