Intrinsic Disorder-Based Design of Stable Globular Proteins.

ABSTRACT: Directed stabilization of globular proteins via substitution of a minimal number of amino acid residues is one of the most complicated experimental tasks. This work summarizes our research on the effect of amino acid substitutions on the protein stability utilizing the outputs of the analysis of intrinsic disorder predisposition of target proteins. This allowed us to formulate the basis of one of the possible approaches to the stabilization of globular proteins. The idea is quite simple. To stabilize a protein as a whole, one needs to find its "weakest spot" and stabilize it, but the question is how this weak spot can be found in a query protein. Our approach is based on the utilization of the computational tools for the per-residue evaluation of intrinsic disorder predisposition to search

SUBMITTER: Nagibina GS 

PROVIDER: S-EPMC7022990 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature