TRIP6 enhances stemness property of breast cancer cells through activation of Wnt/β-catenin.

ABSTRACT:

Background

The urgent problem in the treatment of breast cancer is the recurrence induced by breast cancer stem cells (CSCs). Understanding the role and molecular mechanism of specific molecules in breast cancer stem cells can provide a theoretical basis for better treatment. TRIP6 is an adapter protein which belongs to the zyxin family of LIM proteins and is important in regulating the functions of CSCs. The present study aims to investigate the effects and mechanism of TRIP6 in breast cancer.

Methods

TRIP6 expression in breast cancer cells and tissues were detected by Real-Time PCR, western blot and immunohistochemistry (IHC). MTT assays, colony formation assays, Xenografted tumor model and mammosphere formation assays were performed to investigate the oncogenic functions

SUBMITTER: Zhao X 

PROVIDER: S-EPMC7023708 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature