Diet and Exercise Training Influence Skeletal Muscle Long-Chain acyl-CoA Synthetases.

ABSTRACT:

Introduction

Long-chain acyl-CoA synthetases (ACSL) are implicated as regulators of oxidation and storage of fatty acids within skeletal muscle; however, to what extent diet and exercise alter skeletal muscle ACSL remains poorly understood.

Purpose

This study aimed to determine the effects of diet and exercise training on skeletal muscle ACSL and to examine relationships between ACSL1 and ACSL6 and fat oxidation and fat storage, respectively.

Methods

Male C57BL/6J mice consumed a 60% high-fat diet (HFD) for 12 wk to induce obesity compared with low-fat diet (LFD). At week 4, mice began aerobic exercise (EX-Tr) or remained sedentary (SED) for 8 wk. At week 12, the protein abundance of five known ACSL isoforms and mRNA expression for ACSL1 and ACSL6 were measured in gas

SUBMITTER: Stierwalt HD 

PROVIDER: S-EPMC7024029 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature