Characterization of Pseudomonas sp. TMB2 produced rhamnolipids for ex-situ microbial enhanced oil recovery.

ABSTRACT: The present study describes the ex-situ production of a biosurfactant by Pseudomonas sp. TMB2 for its potential application in enhancing oil recovery. The physicochemical parameters such as temperature and pH were optimized as 30 °C and 7.2, respectively, for their maximum laboratory scale production in mineral salt medium containing glucose and sodium nitrate as best carbon and nitrogen sources. The surface activity of the resulting culture broth was declined from 71.9 to 33.4 mN/m having the highest emulsification activity against kerosene oil. The extracted biosurfactant was characterized chemically as glycolipid by Fourier-transform infrared spectroscopy and ¹H and ¹³C nuclear magnetic resonance spectroscopy analyses. The presence of mono-rhamnolipids (Rha-

SUBMITTER: Haloi S 

PROVIDER: S-EPMC7024075 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature