Effect of Potassium Concentration on Triplex Stability under Molecular Crowding Conditions.

ABSTRACT: The properties of non-canonical DNA structures, like G-quadruplexes and triplexes, change under cell-mimicking molecular crowding conditions relative to dilute aqueous solutions. The analysis of environmental effects on their stability is crucial since they play important roles in gene expression and regulation. In this study, three intramolecular and intermolecular triplex-forming sequences of different C+*G-C triplet content (*: Hoogsteen base pair; - : Watson-Crick base pair) were designed and their stability measured in the absence and presence of a crowding agent with different K+ concentrations. In dilute solution, the stability of the triplexes was reduced by decreasing the concentration of KCl. This reduction became smaller as the number of C+*G-C triplets increased. Under molecula

SUBMITTER: Teng Y 

PROVIDER: S-EPMC7024179 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature