LC-MS/MS-Based Profiling of Tryptophan-Related Metabolites in Healthy Plant Foods.

ABSTRACT: Food plants contain hundreds of bioactive phytochemicals arising from different secondary metabolic pathways. Among these, the metabolic route of the amino acid Tryptophan yields a large number of plant natural products with chemically and pharmacologically diverse properties. We propose the identifier "indolome" to collect all metabolites in the Tryptophan pathway. In addition, Tryptophan-rich plant sources can be used as substrates for the fermentation by yeast strains to produce pharmacologically active metabolites, such as Melatonin. To pursue this technological development, we have developed a UHPLC-MS/MS method to monitor 14 Tryptophan, Tryptamine, amino-benzoic, and pyridine metabolites. In addition, different extraction procedures to improve the recovery of Tryptophan and its deriv

SUBMITTER: Vitalini S 

PROVIDER: S-EPMC7024187 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature