Novel Reporter System Monitoring IL-18 Specific Signaling Can Be Applied to High-Throughput Screening.

ABSTRACT: Very recently, the immunotherapies against cancer, autoimmune diseases, and infection have been feasible and promising. Thus, we have examined the possibility whether or not human gamma delta T cells can be applied for the novel immunotherapies. We previously established the cells stably maintaining NFkB-driven human secreted embryonic alkaline phosphatase (SEAP) expression. The cells can be used to determine the transcription activity of NFkB with high-standard dynamic range and accuracy. Because IL-18 is a kind of cytokines that enhances cytotoxicity and activity of human gamma delta T cells through NFkB activation, we have focused on the activity and signaling of IL-18. In this study, we modified the previous reporter cell that can determine the transcription activity of NFkB to express

SUBMITTER: Kurata R 

PROVIDER: S-EPMC7024245 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature