Lipid and Protein Transfer between Nanolipoprotein Particles and Supported Lipid Bilayers.

ABSTRACT: A nanolipoprotein particle (NLP) is a lipid bilayer disc stabilized by two amphipathic "scaffold" apolipoproteins. It has been most notably utilized as a tool for solubilizing a variety of membrane proteins while preserving structural and functional properties. Transfer of functional proteins from NLPs into model membrane systems such as supported lipid bilayers (SLBs) would enable new opportunities, for example, two-dimensional protein crystallization and studies on protein-protein interactions. This work used fluorescence microscopy and atomic force microscopy to investigate the interaction between NLPs and SLBs. When incubated with SLBs, NLPs were found to spontaneously deliver lipid and protein cargo. The impact of membrane composition on lipid exchange was explored, revealing a positi

SUBMITTER: Dang AT 

PROVIDER: S-EPMC7024587 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature