A Versatile ES Cell-Based Melanoma Mouse Modeling Platform.

ABSTRACT: The cumbersome and time-consuming process of generating new mouse strains and multiallelic experimental animals often hinders the use of genetically engineered mouse models (GEMM) in cancer research. Here, we describe the development and validation of an embryonic stem cell (ESC)-GEMM platform for rapid modeling of melanoma in mice. The platform incorporates 12 clinically relevant genotypes composed of combinations of four driver alleles (LSL-Braf^V600E, LSL-Nras^Q61R, Pten^Flox, and Cdkn2a^Flox) and regulatory alleles to spatiotemporally control the perturbation of genes of interest. The ESCs produce high-contribution chimeras, which recapitulate the melanoma phenotypes of conventionally bred mice. Using the ESC-GEMM platform to modulate Pten expres

SUBMITTER: Bok I 

PROVIDER: S-EPMC7024666 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature