Carbonic anhydrase II does not regulate nitrite-dependent nitric oxide formation and vasodilation.

ABSTRACT:

Background and purpose

Although it has been reported that bovine carbonic anhydrase CAII is capable of generating NO from nitrite, the function and mechanism of CAII in nitrite-dependent NO formation and vascular responses remain controversial. We tested the hypothesis that CAII catalyses NO formation from nitrite and contributes to nitrite-dependent inhibition of platelet activation and vasodilation.

Experiment approach

The role of CAII in enzymatic NO generation was investigated by measuring NO formation from the reaction of isolated human and bovine CAII with nitrite using NO photolysis-chemiluminescence. A CAII-deficient mouse model was used to determine the role of CAII in red blood cell mediated nitrite reduction and vasodilation.

Key results

We found that the c

SUBMITTER: Wang L 

PROVIDER: S-EPMC7024708 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature