Chemistry, pharmacology, and cellular uptake mechanisms of thiometallate sulfide donors.

ABSTRACT:

Background and purpose

A clinical need exists for targeted, safe, and effective sulfide donors. We recently reported that ammonium tetrathiomolybdate (ATTM) belongs to a new class of sulfide-releasing drugs. Here, we investigated the cellular uptake mechanisms of this drug class compared to sodium hydrosulfide (NaHS) and the effects of a thiometallate tungsten congener of ATTM, ammonium tetrathiotungstate (ATTT).

Experimental approach

In vitro H₂ S release was determined by headspace gas sampling of vials containing dissolved thiometallates. Thiometallate and NaHS bioactivity was assessed by spectrophotometry-derived sulfhaemoglobin formation. Cellular uptake dependence on the anion exchange protein (AE)-1 was investigated in human red blood cells. ATTM/glutathion

SUBMITTER: Durham T 

PROVIDER: S-EPMC7024710 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature